Anti-Human CSF2 Recombinant Antibody (TAB-H44)
CAT#: TAB-H44
Recombinant human antibody to Human CSF2
Tested Data
Gene Expression
WB
Figure 1 Anti-Human CSF2 Recombinant Antibody (TAB-H44) in WB
Western blot analysis of TAB-H44 with human CSF2.
Lane1: Reducing antigen (0.1 μg)
Lane2: Reducing antigen (0.3 μg)
Lane3: Reducing antigen (0.6 μg)
ELISA
Figure 2 Anti-Human CSF2 Recombinant Antibody (TAB-H44) in ELISA
ELISA analysis of MRO-1214LC was performed by coating with human CSF2.
SDS-PAGE
Figure 3 Anti-Human CSF2 Recombinant Antibody (TAB-H44) in SDS-PAGE
SDS-PAGE analysis of TAB-H44 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
SEC-HPLC
Figure 4 Anti-Human CSF2 Recombinant Antibody (TAB-H44) in SEC-HPLC
SEC-HPLC analysis of TAB-H44 shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57404/1703_H9_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (BJ hTERT+)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164400-CSF2
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Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG1 - kappa
- Specificity
- CSF2 (colony stimulating factor 2 (granulocyte-macrophage), granulocyte-macrophage colony stimulating factor, GM-CSF) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- Neut, ELISA, IF, IP, FuncS, FC, IHC, Block
- Related Disease
- Inflammatory diseases
Product Property
- Purity
- >95.0% as determined by analysis by SDS-PAGE.
Applications
- Application Notes
- The CSF2 antibody has been reported in applications of Neut, ELISA, IF, IP, FuncS, FC, IHC, Block.
Target
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(Creative Biolabs Cat# TAB-H44, RRID: AB_3112031)
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Biosimilar Overview
Please refer to Lenzilumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Lenzilumab.
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1257z | Mouse Anti-CSF2 Recombinant Antibody (clone 21C4) | WB, ELISA, IHC, FuncS | Mouse IgG1 |
| MOB-1737MZ | Recombinant Mouse Anti-Human CSF2 Antibody (clone 230) | ELISA | Mouse antibody |
| PABC-337 | Human Anti-CSF2 Recombinant Antibody (clone F1) | ELISA, FC, Neut | Human IgG |
| HPAB-N0052-YC | Human Anti-CSF2 Recombinant Antibody (HPAB-N0052-YC) | ELISA, Block | Humanized IgG |
| ZG-0390J | Mouse Anti-CSF2 Recombinant Antibody (ZG-0390J) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-259 | Anti-Human CSF2 Recombinant Antibody (TAB-259) | IP, IF, FuncS, FC, Neut, ELISA, ICC | IgG1 - kappa |
| TAB-177CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (KB002) | ELISA, Neut | Human antibody |
| TAB-178CT | Human Anti-CSF2 Recombinant Antibody (TAB-178CT) | ELISA, Neut, FC, WB | Human IgG1, κ |
| TAB-179CT | Human Anti-CSF2 Recombinant Antibody (TAB-179CT) | ELISA, WB, Neut | Human IgG1, κ |
| TAB-180CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (F1) | Neut, Inhib, FC, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-183CT | Mouse Anti-CSF2 Recombinant Antibody (TAB-183CT) | ELISA, FC, WB, Block | Mouse IgG |
| TAB-185CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (4K21) | ELISA, Neut | |
| TAB-183CT-S(P) | Mouse Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-183CT-S(P)) | ELISA, FC, WB, Block | Mouse scFv |
| TAB-185CT-S(P) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody scFv Fragment (4K21) | ELISA, Neut | |
| TAB-183CT-F(E) | Mouse Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-183CT-F(E)) | ELISA, FC, WB, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-184CT | Human Anti-CSF2 Recombinant Antibody (TAB-184CT) | ELISA, FC, WB, Block | Chimeric (Mouse/Human) IgG1, κ |
| TAB-184CT-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (TAB-184CT-S(P)) | ELISA, FC, WB, Block | Human scFv |
| TAB-184CT-F(E) | Human Anti-CSF2 Recombinant Antibody; Fab Fragment (TAB-184CT-F(E)) | ELISA, FC, WB, Block | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-186CT | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (hGM4/1-34) | ELISA, Neut | Humanized antibody |
| TAB-186CT-S(P) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody scFv Fragment (hGM4/1-34) | ELISA, Neut | Humanized antibody |
| TAB-186CT-F(E) | Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody Fab Fragment (hGM4/1-34) | ELISA, Neut | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-511CQ | Recombinant Mouse Anti-CSF2 Antibody (CBL568) | Neut | IgG1 |
| NEUT-513CQ | Rat Anti-CSF2 Recombinant Antibody (clone 23B6) | Neut | Rat IgG2a |
| NEUT-515CQ | Mouse Anti-CSF2 Recombinant Antibody (NEUT-515CQ) | IHC, Neut, WB | Mouse IgG1 |
| NEUT-516CQ | Mouse Anti-CSF2 Recombinant Antibody (clone BVD2-21C11) | Neut, FC, ICC, IF, WB, IP, IHC-Fr | Mouse IgG2a, κ |
| NEUT-517CQ | Rat Anti-CSF2 Recombinant Antibody (clone BVD2-23B6) | ELISA, FC, IP, Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-512CQ | Rat Anti-CSF2 Recombinant Antibody (clone 22E9), Biotin | Block, ELISA, WB | Rat IgG2a |
| NEUT-514CQ | Rat Anti-CSF2 Recombinant Antibody (clone 31G6) | Block, ELISA, WB | Rat IgG1 |
| NEUT-525CQ | Rat Anti-Csf2 Recombinant Antibody (clone CBL251) | Inhib | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4580 | Hi-Affi™ Rabbit Anti-CSF2 Recombinant Antibody (clone TH92DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0014-FY-F(E) | Human Anti-CSF2 Recombinant Antibody Fab Fragment (HPAB-0014-FY-F(E)) | ELISA | Human Fab |
| HPAB-0136-FY-F(E) | Human Anti-CSF2 Recombinant Antibody (clone MSB-0010628); Fab Fragment | Inhib | Humanized Fab |
| PFBC-337 | Human Anti-CSF2 Recombinant Antibody (clone F1); Fab Fragment | ELISA, FC, Neut | Human Fab |
| PFBC-338 | Mouse Anti-CSF2 Recombinant Antibody (clone 4D4); Fab Fragment | ELISA, FC, Neut | Mouse Fab |
| HPAB-N0052-YC-F(E) | Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-N0052-YC-F(E)) | ELISA, Block | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-337 | Human Anti-CSF2 Recombinant Antibody (clone F1); scFv Fragment | ELISA, FC, Neut | Human scFv |
| PSBC-338 | Mouse Anti-CSF2 Recombinant Antibody (clone 4D4); scFv Fragment | ELISA, FC, Neut | Mouse scFv |
| HPAB-N0052-YC-S(P) | Human Anti-CSF2 Recombinant Antibody; scFv Fragment (HPAB-N0052-YC-S(P)) | ELISA, Block | Humanized scFv |
| HPAB-1090-FY-F(E) | Human Anti-CSF2 Recombinant Antibody; Fab Fragment (HPAB-1090-FY-F(E)) | ELISA | Human Fab |
| PABJ-0112-S(P) | Human Anti-CSF2 Recombinant Antibody scFv Fragment (PABJ-0112-S(P)) | ELISA, Neut | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-259 | Afuco™ Anti-CSF2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-259) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| AFC-TAB-H44 | Afuco™ Anti-CSF2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H44) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY70 | AbPlus™ Anti-CSF2 Magnetic Beads (BVD2-21C11) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY98 | Human Anti-CSF2 (clone MSB-0010628) scFv-Fc Chimera | Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1707 | Anti-CSF2 Immunohistochemistry Kit | IHC |
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